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Liquid Chromatography

Accurate Analysis Starts With Chromatography

A sample is injected into the chromatographic system and carried by the mobile phase through the column, where its components interact with the stationary phase. Molecules with weaker interactions with the stationary phase elute more quickly, while those with stronger interactions are retained longer. As each component exits the column, it is detected by one or more suitable detectors. Common options include UV-Vis, photodiode array (PDA), fluorescence, evaporative light scattering detector (ELSD), charged aerosol detector (CAD), and electrochemical or pulsed amperometric detectors (PAD). Each detector provides a signal that is generally proportional to analyte concentration. Under fixed chromatographic conditions, the unique retention time of each component, together with its detector response, supports both qualitative identification and quantitative measurement of analytes.
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Chromatography-1

Detectors
Available

UV-Vis

PDA

Fluoresecence 

Refractive Index

ELSD

Electrochemical/Pulsed Ampeometric

charged aerosol detector

MS

How Liquid Chromatography Works

A sample is injected and carried by the mobile phase through the column. Components with weaker interaction with the stationary phase elute faster, while those that bind more strongly linger longer. As each component exits the column, detectors like UV-Vis, PDA, fluorescence, or ELSD record signals proportional to concentration. The unique retention time under the fixed chromatographic conditions helps identify each analyte.

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Application

Practical Uses of Liquid Chromatography:

  • Analyze purity, impurities, and compounds in raw or finished products
  • Perform reverse engineering or deformulation of competitor products
  • Support R&D, method development, and validation for new formulations

Benefits

Why This Technique Matters:

  • Deliver precise separation of compounds in complex mixtures
  • Provide reliable data for identity and comparison to standards
  • Adapt detection methods (UV, fluorescence, CAD) for chemical types


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